Skip to main content
. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Microbiology (Reading). 2007 Jun;153(Pt 6):1799–1807. doi: 10.1099/mic.0.2007/005975-0

Fig. 3.

Fig. 3

Effect of a comD mutation on comC and nlmC gene expression. (a) Expression of comC in wild-type and comD mutant backgrounds with and without CSP. Transcript levels were measured by real-time RT-PCR using 16S RNA as a housekeeping control. cDNA abundance was normalized against the 16S cDNA and expressed as relative cDNA abundance. The wild-type level without CSP was arbitrarily set as 1. (b) nlmC expression in wild-type and comD mutant backgrounds with and without CSP. Transcript levels were measured using the nlmC–luc reporter and normalized against cell density. Values and standard deviation are calculated from at least two independent experiments performed in triplicate.