Table 1.
Schematic display of the Aas I and Dra I digestion of phNIS-IRES-EGFP. The table summarizes the size of the plasmid DNA fragments of the phNIS-IRES-EGFP and its transport form Clamp-PNA-BioShuttle-phNIS-IRES-EGFP after cleavage with restriction enzymes Aas I and Dra I.
| phNIS -IRES-EGFP | BioShuttle-phNIS-IRES-EGFP | ||||
|---|---|---|---|---|---|
| expected fragments | calculated fragments | fragments after digestion with DraI and AasI | expected fragments | calculated fragments | fragments after digestion with DraI and AasI |
| 1957 | 1957 = 1588 + 369 | 1957 | 1957 = 1588 + 369 | ||
| 1789 | 1789 = 1117 + 653 + 19 | 1789 | 1789 = 1117 + 653 + 19 | ||
| 1707 | 1707 | 1707 | 1707 | 1707 | 1707 |
| - | 1588 | 1588 | 1588 | 1588 | 1588 |
| 1117 | 1117 | 1117 1136 = 1117 + 19 | 1117/1136 | 1117 | 1117 1136 = 1117 + 19 |
| 1062 | 1062 | 1062 | 1062 | 1062 | 1062 |
| 956 | 956 | 956 | 956 | 956 | 956 |
| 653 | 653 | 653 672 = 653 + 19 | - | 653 + BioShuttle | 653 672 = 653 + 19 + BioShuttle |
| 369 | 369 | 369 | 369 | ||
| 19 | 19 | 19 | 19 | ||