TABLE 3.
Cytokine/Chemokine Release of PHA/PMA-Stimulated Jurkat Human T-Cell Leukemia Cells and TNFα-Stimulated A549 Cells
| ExcelArray Inflammation I | IFNγ | IL-1β | IL-2 | IL-4 | IL-6 | IL-8 | IL-10 | IL-12p70 | RANTES | MIP-1α | MIP-1β | TNFα |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Jurkat + PHA/PMA | — | — | >1000 | — | — | >1000 | — | — | — | >1000 | 169 | 770 |
| A549 + TNFα | — | — | — | — | 80 | 26,000 | — | — | 17,000 | — | 50 | — |
| ExcelArray Inflammation II | IL-1α | IL-3 | IL-5 | IL-7 | IL-13 | IL-15 | IL-17 | FasL | G-CSF | GM- CSF | TNFβ | MCP-1 |
| Jurkat + PHA/PMA | — | >1000 | — | — | 400 | — | — | 40 | 105 | >1000 | 419 | — |
| A549 + TNFα | — | — | — | — | — | — | — | — | 120 | — | — | 18,000 |
| ExcelArray Angiogenesis | Angiogenin | EGF | FGFb | GROα | HGF | IL-8 | HB- EGF | KGF | PlGF | VEGF | TIMP-1 | TNFα |
| A549 + TNFα | — | — | — | 38,000 | — | 26,000 | — | — | — | — | 21,000 | — |
| ExcelArray Chemotaxis | Eotaxin | I-309 | IP-10 | MCP-3 | MDC | IL-8 | Fractalkine | TARC | RANTES | MIP-1α | MIP-1β | MCP-1 |
| Jurkat + PHA/PMA | — | >1000 | 93 | — | 95 | >1000 | — | — | — | >1000 | 169 | — |
| A549 + TNFα | — | — | 490 | — | 250 | 26,000 | — | 130 | 17,000 | — | 50 | 18,000 |
Analytes detected at an increased level in stimulated cell culture supernatants as compared to non-stimulated controls are listed. Values listed are the difference of stimulated cell culture supernatant analyte concentration minus non-stimulated cell culture supernatant analyte concentration.