Table 4.
PA Domain Binding by PA-specific Fabs
| Capture assay |
Western blots |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Clone | VL | VH | PA83a | PA63a | PA20a | fD4b | fD1c | fD4b | PrFraga |
| 11A11 | IGKV1-5 | IGHV1-18 | + | − | + | nd | + | − | - |
| 4A12 | IGKV1D-39 | IGHV1-46 | + | + | − | + | − | + | PA63 |
| 35F7 | IGLV1-40 | IGHV3-15 | + | − | +/− | − | + | − | PA20 |
| 40D10 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 59H10 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 46D1 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 41A3 | IGLV1-40 | IGHV3-15 | + | − | +/− | − | + | − | PA20 |
| 49F1 | IGLV1-40 | IGHV3-15 | + | − | − | − | +/− | − | - |
| 55C7 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | nd |
| 52E7 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | nd |
| 66A5 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 34E3 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 41C5 | IGLV1-40 | IGHV3-15 | + | − | +/− | − | + | − | PA20 |
| 51D6 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 53H4 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 54C7 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | nd |
| 56F6 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 65A8 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | nd |
| 65H8 | IGLV1-40 | IGHV3-15 | + | − | + | nd | + | − | PA20 |
| 65G8 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | PA20 |
| 66B2 | IGLV1-40 | IGHV3-15 | + | − | + | − | + | − | nd |
| 17D2 | IGKV1-9 | IGHV3-30 | + | + | − | − | − | − | PA63 |
| 65A3 | IGLV3-1 | IGHV3-30 | + | − | +/− | − | + | − | nd |
| 47F12 | IGLV1-44 | IGHV3-30 | + | − | + | − | + | − | PA20 |
| 59G5 | IGLV1-44 | IGHV3-30 | + | − | + | − | + | − | PA20 |
| 8H2 | IGKV6-21 | IGHV3-33 | + | − | + | − | + | − | PA20 |
| 19E1 | IGKV6-21 | IGHV3-33 | + | − | + | nd | + | − | PA20 |
| 41F12 | IGLV1-47 | IGHV3-33 | + | − | + | − | + | − | PA20 |
| 55F9 | IGLV1-47 | IGHV3-33 | + | − | + | − | + | − | PA20 |
| 20C7 | IGKV1-9 | IGHV3-43 | + | + | − | + | − | + | - |
| 51C5 | IGLV3-21 | IGHV3-74 | + | − | + | − | − | − | - |
| 1A5 | IGKV2-30 | IGHV4-59 | + | + | − | − | − | − | PA63 |
| 1B10 | IGKV2-30 | IGHV4-59 | + | +/− | − | − | − | − | PA63 |
| 4B1 | IGKV2-30 | IGHV4-59 | + | − | − | − | − | − | nd |
| 13E12 | IGKV2-30 | IGHV4-59 | + | +/− | − | − | − | − | nd |
a
Purified PA83 or purified PA63 or PA20 proteolytic fragments.
b
Purified Domain 4-GFP fusion protein.
c
Purified Domain 1(PA20)-GFP fusion protein.