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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Gene. 2007 Aug 9;402(1-2):94–102. doi: 10.1016/j.gene.2007.07.027

Figure 3. Trans-activation of the HiNF-P Promoter by multiple gene regulatory factors.

Figure 3

(A) The bar graph shows luciferase results obtained upon co-transfection of the full length HiNF-P promoter/Luciferase construct and expression vectors for the gene regulatory factors HiNF-P, SP1, E2F, p220NPAT, p300 and CBP as indicated. The reporter gene constructs (100 ng) and expression vector (200 ng) were transiently transfected in triplicate into SAOS-2 cells. (B) Western blot analysis of luciferase cell lysates for each of the factors expressed in Panel A (+; right lanes) relative to lysates from control cells transfected with empty expression vectors (−; left lanes). Each lysate was analyzed with the indicated antibodies to detect endogenous versus exogenous expression levels of HiNF-P, SP1, E2F, p220NPAT, p300 or CBP. The nuclear protein Lamin B was used as an internal control for protein quantitation.