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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Gene. 2007 Aug 9;402(1-2):94–102. doi: 10.1016/j.gene.2007.07.027

Figure 6. Delineation of HiNF-P promoter elements responding to p220NPAT, p300 and CBP.

Figure 6

Transcription assays were performed with SAOS-2 and WI-38 cells as described in Figure 5 using a panel of Luciferase constructs (100 ng) fused to the indicated HiNF-P promoter deletions and expression vectors (200 ng each) for p220NPAT (A), p300 and CBP (B). Luciferase values were normalized to the respective controls which were set as one. (C) Pathways converging on the HiNF-P promoter identified in this study are indicated. The proximal promoter responds to the levels of SP1 and E2F that each have known consensus motifs. The HiNF-P promoter is also stimulated by the co-activators p300 and CBP that are known to control histone acetylation. Both proteins may interact with the HiNF-P promoter through p220NPAT. HiNF-P also enhances the activity of its own promoter but motifs for HiNF-P are not readily apparent in the promoter.