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. Author manuscript; available in PMC: 2008 Sep 30.

Published in final edited form as: J Immunol Methods. 2007 Jul 18;326(1-2):33–40. doi: 10.1016/j.jim.2007.07.003

Fig. 5 — (A) Detection of phage peptide-specific IgG in MS CSF by RT-IPCR. MS CSF (03−1), CSF from an inflammatory control patient (IC, Cryptococcal meningitis), both at IgG concentrations of 5 μg/ml, and BSA were added to ELISA plates coated with anti-human IgG. Phage 2−6−4 (specific for rAb #37) at concentrations of 10⁹, 10⁸ and 10⁷ were added to each well. Bound phage was collected and quantified by (RT) PCR as described in Fig. 2A. Phage peptide 2−6−4 bound specifically to IgG in MS CSF, but not to IC control CSF or to BSA. Error bars represent standard deviation of duplicate samples. (B) MS CSF screening for phage-specific IgG using RT-IPCR. Eight MS CSFs and 7 inflammatory control CSFs were screened with phage 2−6−4 (5 × 10⁸) for IgG reactivity as described in Fig 5A. Phage 2−6−4 bound only to MS 03−1 CSF (MS 8) from which rAb #37 was cloned, but not to any other MS CSFs or the IC CSF. Error bars represent standard deviation of duplicate samples.

Fig. 5 — (A) Detection of phage peptide-specific IgG in MS CSF by RT-IPCR. MS CSF (03−1), CSF from an inflammatory control patient (IC, Cryptococcal meningitis), both at IgG concentrations of 5 μg/ml, and BSA were added to ELISA plates coated with anti-human IgG. Phage 2−6−4 (specific for rAb #37) at concentrations of 10⁹, 10⁸ and 10⁷ were added to each well. Bound phage was collected and quantified by (RT) PCR as described in Fig. 2A. Phage peptide 2−6−4 bound specifically to IgG in MS CSF, but not to IC control CSF or to BSA. Error bars represent standard deviation of duplicate samples. (B) MS CSF screening for phage-specific IgG using RT-IPCR. Eight MS CSFs and 7 inflammatory control CSFs were screened with phage 2−6−4 (5 × 10⁸) for IgG reactivity as described in Fig 5A. Phage 2−6−4 bound only to MS 03−1 CSF (MS 8) from which rAb #37 was cloned, but not to any other MS CSFs or the IC CSF. Error bars represent standard deviation of duplicate samples.