Table 1. Microarray and qRT-PCR analysis for expressed and non-expressed genes in periglomerular cells1 .
Gene | Signal detection value | Ct 2 |
Actb | 18925 | 17.72 |
Egfp | -3 | 18.17 |
Htr3a | 607 | 21.12 |
Dcx | 11202 | 22.80 |
Gad1 | 28449 | 19.50 |
Mog | <100 | 29.53 |
Omp | <100 | 30.78 |
Csf1 | 161 | 30.09 |
Syn1 | 269 | 32.02 |
Gria3 | 277 | 28.21 |
sequences of primers used for qRT-PCR are shown in Table 2.
Ct–cycle of detection of amplifying PCR product.
Egfp gene cannot be detected by Affymetrix Gene Chip Mouse Genome Array.