. Author manuscript; available in PMC: 2008 Aug 15.

Published in final edited form as: J Neurosci Methods. 2007 Apr 24;164(1):149–154. doi: 10.1016/j.jneumeth.2007.04.012

Table 1.

Real-time PCR primers used to assay vasopressin transcription.

Primers	Sequence	Product	T_A	T_M
VP Intron 2 forward	5′-GCAGGGCTGAAGGGAATCCAGTCA	171 bp	66 °C, 30 s	83.3 °C
VP Intron 2 reverse	5′-TTAGGCTGATGGGTGCAAGACACA

VP Exon 1 forward	5′-TGCCTGCTACTTCCAGAACTGC	77 bp	60 °C, 30 s	81.2 °C
VP Exon 1/2 bridge rev.	5′-AGGGGAGACACTGTCTCAGCTC

T7 forward	5′-TAATACGACTCACTATAGGG	220_i,130_e bp	50 °C, 30 s	-

T_A, annealing temperature; T_M, PCR product melting temperature; 220_i and 130_e products from separate plasmids using the T7 forward primer with either VP Intron 1 reverse or Exon 1/2 bridge reverse primers.