Figure 3.

Genetic code alterations induce ploidy variation. Flow cytometry analysis of haploid (A) and diploid (B) S. cerevisiae cell lines expressing the C. albicans G₃₃ or U₃₃ tRNA_CAG^Ser had a general increase of the nuclear DNA content, providing evidence of polyploidy and aneuploidy events in CUG ambiguous cells. (C) Ploidy shift was observed in 56% of the haploid G₃₃ tRNA_CAG^Ser clones and 50% of diploid U₃₃ tRNA_CAG^Ser clones tested by flow cytometry analysis. (D) Heterogeneity of the ambiguous cell population is shown by the variability in colony, cell and bud size and shape. The increase in cell volume is consistent with polyploidization of the ambiguous clones. DAPI staining highlights ambiguous cells with two nuclei or without nucleus, suggesting the presence of polyploid and aneuploid cells.