Figure 6.

Genetic code alterations increase trehalose and glycogen accumulation. C and U₃₃ represent S. cerevisiae control cells and cells expressing C. albicans U₃₃ tRNA_CAG^Ser, respectively. The results are expressed as mean±s.d. of four independent experiments (^*P<0.05 and ^**P<0.01 by Student's t-test). Trehalose (A) and glycogen (B) content were 6 fold higher in exponentially growing CUG ambiguous cells (OD=0.5), when compared with control cells. (C, D) Glycogen and trehalose accumulation were induced in ambiguous cells after a 30-min heat shock; glycogen content increased 8 fold under heat shock when compared with control cells, while trehalose accumulation increased in both ambiguous and control strains under heat stress.