Table 2.
Plasmid list
| Strain | Relevant characteristics | Source or reference |
|---|---|---|
| pRS415 | CEN LEU2 | Sikorski and Hieter (1989) |
| pRS416 | CEN URA3 | Sikorski and Hieter (1989) |
| pRS426 | 2μURA3 | Sikorski and Hieter (1989) |
| pEG202/3 | 2μHIS3 ADH1-LexA-DBD | Gyuris et al (1993) |
| pJG4-5/6 | 2μTRP1 GAL1,10-AD | Gyuris et al (1993) |
| pRD53 | CEN URA3 GAL1,10 | R Deshaies |
| pMG180 | pRS416 GAL1,10 GFP-BEM2 | This study |
| pNP337 | pRS416 GFP-Bem3 | This study |
| pMG184 | pRS416 ADH1 GFP-BEM2 | This study |
| pMG220 | pRS416 BEM3-myc | This study |
| pMK51 | pRS416 BEM3-S3A-myc | This study |
| pMK81 | pRS416 BEM3-S5A-myc | This study |
| pMG230 | pRS416 BEM3-R950G-myc | This study |
| pMG231 | pRS416 BEM3-R950K-myc | This study |
| pYS417 | pRS415 GAL1,10 GFP-BEM3 | This study |
| pYS418 | pRS415 GAL1,10 GFP-BEM3-R950G | This study |
| pYS419 | pRS415 GAL1,10 GFP-BEM3-R950K | This study |
| pMK61 | pRS415 GALS GFP-BEM3 | This study |
| pMK70 | pRS415 GALS GFP-BEM3-S3A | This study |
| pMK91 | pRS415 GALS GFP-BEM3-S5A | This study |
| pMK101 | pRS415 GAL1,10 GFP-BEM3-5A- R950G | This study |
| pMK187 | pRS416 GALS GFP-BEM3 | This study |
| pYS23 | pRS415 ADH1 CDC24-GFP | Shimada et al (2000) |
| pMJ383 | pRS314 GAL1,10 GIC2-GFP | Jaquenoud and Peter (2000) |
| pMG323 | pEG202 CDC42-C188S | Butty et al (1998) |
| pMG362 | pEG203 CDC42-Q61L-C188S | This study |
| pSS5 | pJG4-6 BEM3 | This study |
| pMG214 | pJG4-6 BEM3-R950G | This study |
| pMG215 | pJG4-6 BEM3-R950K | This study |
| pYS82 | pRD53 ADH FAR1-22 | This study |
| pMG201 | pET30A 6HIS-BEM3 (1-353) | This study |
| pMK195 | pRS415 GAL1,10 BEM3-FLAG | This study |