Figure 4.
Xorbit is required to maintain kinetochore and nonkinetochore MTs at anaphase onset. (A) Wild-type metaphase spindles were assembled, and GST or GST-CT was added at the same time anaphase was induced. Spindles were fixed 5, 10, and 15 min later. (B) Wild-type spindles were assembled around chromatin-coated beads, and GST or GST-CT was added during metaphase arrest (bottom left) or at the same time anaphase was induced (right), and spindles were fixed 10 min later. MTs are red and DNA is blue. Bars, 10 μm.