Skip to main content
. 2006 Jan 2;172(1):55–66. doi: 10.1083/jcb.200510016

Figure 7.

Figure 7.

Loss of SRO7 exacerbates the sec3Δ phenotype. (A) Wild-type (WT), sro7Δ, sec3Δ, and sec3Δ sro7Δ yeast strains were spotted onto SC media plates in 10-fold dilutions and grown at the indicated temperatures. (B and C) Wild-type, sec3Δ, and sec3Δ sro7Δ yeast strains were grown at 30°C in SC media. (B) The doubling time was measured by plotting OD600 over time. The mean of three independent experiments is shown. (C) Deletion of SRO7 in a sec3Δ strain leads to a more pronounced defect in exocytosis. Expression of the enzyme invertase was derepressed by shifting the strains into low glucose medium, and secretion of invertase was measured after 1 h of derepression by a colorimetric test (see Materials and methods). Values indicate percentages of secreted invertase. A mean of four different experiments is shown. Error bars represent SEM.