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. 2006 Jan 2;172(1):91–102. doi: 10.1083/jcb.200508044

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Figure 1. — Pax3 expression in muscle satellite cells. (A–C) Expression of Pax3 in different muscles from 3-wk-old Pax3 ^IRESnLacZ/+ mice, revealed by X-Gal staining. (A) Diaphragm muscle; (B) hindlimb muscles, (g) gracilis muscle; (C) trunk muscles, (sdc) serratus dorsalis caudalis. (D) Semiquantitative RT-PCR of Pax3 and -7 transcripts in adult tibialis anterior (TA) and diaphragm muscle. The number of cycles is indicated on the top of each lane. M, molecular weight markers (see Materials and methods). (D') Pax3 protein is detected by Western blot in protein extracts from different muscles (D, diaphragm; L, hindlimb; T, ventral trunk muscles) in 3-wk-old mice. Tubulin (Tub) expression is shown as a loading control. (E and F) Pax3 ^IRESnLacZ/+ is expressed in a subset of diaphragm muscle nuclei (arrows) from 3-wk-old mice, as revealed by X-Gal and DAPI staining of a transverse section (E) or isolated fiber (F). (G and G') Detection of Pax3/7-dependent β-gal expression in transverse sections of adult diaphragm muscle from the transgenic line P34, which reports Pax3/7 transcriptional activity. (G) Immunodetection of laminin (red) and DAPI (blue) staining. (G') Coimmunodetection of laminin (red) and β-gal (green). (H–J') Coimmunodetection of β-gal–positive cells in the diaphragm muscle of 3-wk-old Pax3 ^IRESnLacZ/+ mice with laminin (H', green), CD34 (I', red), or M-cadherin (J', red). As indicated in the figure, β-gal is shown in red in H' and in green in I' and J'. Corresponding DAPI staining is shown (H, I, and J) for each panel. Arrows indicate the labeled satellite cell nuclei. (K) Coimmunodetection of Pax3 (red) and laminin (green, K'). Corresponding DAPI staining is shown, with the labeled nucleus indicated by an arrow (K). (L–N') Coimmunohistochemistry on diaphragm muscle from 3-wk-old Pax3 ^IRESnLacZ/+ mice for Pax7 (red) or β-gal (green). (L–N) Corresponding DAPI staining is presented with labeled nuclei indicated by arrows for each panel.