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. 2006 Jan 2;172(1):91–102. doi: 10.1083/jcb.200508044

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Figure 4. — Pax3 expression is maintained in Pax7-deficient satellite cells. (A) Western blot analysis of Pax3 expression in the diaphragm of Pax7 ^LacZ/+ (+/−) or Pax7 ^LacZ/LacZ (−/−) mice at P3. Tubulin (Tub) expression is shown as a loading control. (B) Coimmunohistochemistry on transverse sections of ventral trunk muscle of Pax7 ^LacZ/LacZ mice at P2 using DAPI staining and an antibody that recognizes Pax3. Laminin staining shows a Pax3-positive cell (red) present in a satellite cell position. (C) Immunohistochemistry on transverse sections of ventral trunk muscle from Pax7 ^LacZ/+ or Pax7 ^{LacZ/ LacZ} mice at P2 using antibodies recognizing β-gal (green) and laminin (red). Corresponding DAPI staining is indicated. Arrowheads indicate Pax7 (β-gal)–expressing cells, located under the basal lamina. (D) Coimmunocytochemistry on 7-d primary cultures derived from the diaphragm of Pax7 ^LacZ/+ or Pax7 ^LacZ/LacZ mice at P10 using antibodies recognizing MyoD and troponin T. (E) Quantification of the number of β-gal–positive satellite cells in trunk, diaphragm (dia), and hindlimb (limb) muscles of Pax7 ^LacZ/+ (+/−) or Pax7 ^LacZ/LacZ (−/−) mice, detected per fiber on 10-μm sections from ventral trunk muscle at P2 or P11. (F) Infection of primary cultures from Pax7 ^LacZ/LacZ mutant mice (Pax7 ⁻ ^/ ⁻) at P4 with an adenovirus expressing a dominant-negative form of Pax3 (Adeno GFP+Pax3DN) shows elimination of MyoD expression in infected cells (right arrowhead, infected cell; left arrowhead, noninfected cell), whereas virus expressing GFP alone had no effect. Quantification is shown in G. No MyoD-positive myogenic cells are present after Pax3DN infection.