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. 2006 Jan 2;172(1):151–162. doi: 10.1083/jcb.200506184

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Figure 1. — Cre efficiently mediates gene excision early in the vasculature of Tie2-C re embryos. (A and B) AP-stained embryos at e7.5 (A) and e8.5 (B) show Cre activity in blood islands (arrows) and dorsal aortae (arrowheads). (C) A LacZ-stained YS at e11.5. (D) Paraffin section of e11.5 LacZ-stained embryo shows Cre activity in ECs (arrowhead) and blood cells (arrow). (E and F) FAK expression in cells from e9.5 embryos stained with anti-CD31 (green), DAPI (blue), and anti-Y397pFAK (red, E and F) or anti-FAK (red, E' and F'). Bars, 10 μm. (G) Cre-mediated FAK depletion was measured by the percentage of FAK⁻ CD31⁺ cells in mutants at e8.5, 9.5, and 10.5 relative to controls. Each represents the mean ± SEM (error bars) of 100 CD31⁺ cells in three replicate (for anti-Y397FAK) and five replicate (for anti-FAK) experiments. (H) Expression of FAK, Pyk2, and FRNK by Western or IP analyses of e9.5 embryos.