Figure 3.

Sequences of the junction of NHEJ events at locus L1 in Δnup145C and proposed mechanisms of NHEJ. Coordinates of the L1 locus on chromosome XI-L are shown. I-SceI restriction sites (in opposite orientations) are in bold, cleavage position generating 4-bp 3′ overhangs is indicated by a staggered line, BamHI and KpnI restriction sites are boxed and plasmid sequences are in lower case letters (see Fairhead et al., 1996 for construction details). Possible end processing and subsequent alignment of complementary base pairs are shown. See text for details.