Table II. Strains used in this work.
| Strain name | Genotype | Reference |
|---|---|---|
| FYBL1-8B/Ai0611 | MATa, ura3-Δ851, leu2Δ1, his3Δ200, lys2Δ202, ykl222c::csURA3csa | (Ricchetti et al., 2003) |
| YEF402 | FYBL1-8B/Ai0611/Δnup145C::kanMX4 | This work |
| YPT005 | MATa, ura3, trp1-Δ63, leu2, his3, ykl222c::csURA3cs, Δnup84::kanMX4b | This work |
| YPT009 | MATa, ura3, trp1-Δ63, leu2, his3, ykl222c::csURA3cs, Δnup170::kanMX4b | This work |
| YPT084 | MATa, ura3, trp1-Δ63, leu2, his3, ykl222c::csURA3cs, Δnup133::kanMX4b | This work |
| YPT090 | FYBL1-8B/Ai0611/Δnup120::kanMX4 | This work |
| FYBL1-8B/Bn001 | MATa, ura3-Δ0, leu2Δ1, his3Δ200, lys2Δ202, ykl201c::csURA3cs | (Ricchetti et al., 2003) |
| YEF460 | FYBL1-8B/Bn001/Δnup145C::kanMX4 | This work |
| YPT007 | MATa, ura3, trp1-Δ63, leu2, his3 ykl201c::csURA3cs, Δnup84::kanMX4b | This work |
| YPT012 | MATa, ura3, trp1-Δ63, leu2, his3, Δnup170::kanMX4b | This work |
| YW656 | Matα, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, can1Δ::ade2-K1, ade2::SD2-::GAL-IsceI:: URA3 |
(Karathanasis and Wilson, 2002) |
| YEF458 | YW656/Δnup145C::kanMX4 | This work |
| YW 969 | Matα, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, can1Δ::ade2-K1, ade2::SD2-::GAL-IsceI:: URA3 |
(Karathanasis and Wilson, 2002) |
| YEF456 | YW969/Δnup145C::kanMX4 | This work |
| YEF472a | MATa, ura3Δ851, leu2Δ1, his3Δ200, lys2Δ202, ykl222c::csURA3cs::tet0112::NATMX, LEU2::tetR-GFP |
This work |
| YEF473a | YEF472a/Δnup145C::kanMX4 | This work |
| YEF499-8A | YEF472a/Δku70::kanMX4 | This work |
| YPT030 | MATa, ura3, leu2, his3, lys2, ykl222c::csURA3cs::tet0112::NATMX, LEU2::tetR-GFP, Δnup84::kanMX4b |
This work |
| YPT082 | YEF472a/Δnup133::kanMX4 | This work |
| YPT087 | YEF472a/Δnup120::kanMX4 | This work |
| YPT065 | MATa ura3, trp1Δ63, leu2, his3, lys2, ade2Δ, ykl222c::csURA3cs::tet0112::NATMX, LEU2::tetR-GFP, Δesc1::TRP1b |
This work |
| YPT071 | YEF472a/Δsir4::kanMX4 | This work |
| YEF548 | MATa, ura3-Δ851, leu2Δ-1, his3Δ200, lys2Δ-202, ykl222c::csURA3cs::tet0112:: NATMX, LEU2::tetR-GFP, Δnup145C::KANMX4, Δku70::hph |
This work |
| FEP100 | MATa, ura3-52, leu2Δ1, ade2Δ, can1-1, tel XI coreX::URA3 | (Pryde and Louis, 1999) |
| YEF486-6C | FEP100/Δnup145C::kanMX4 | This work |
| YPT001 | MATα, ura3, leu2, ade2Δ, telXI coreX::URA3, Δnup84::kanMX4b | This work |
| YPT003 | MATα, ura3, leu2, ade2Δ, telXI coreX::URA3, Δnup170::kanMX4b | This work |
| YPT0034 | MATa, ura3, leu2, ade2Δ, telXI coreX::URA3, Δnup53::kanMX4b | This work |
| YPT036 | MATα, ura3, leu2, ade2Δ, telXI coreX::URA3, Δnup120::kanMX4b | This work |
| YPT038 | MATα, ura3, leu2, ade2Δ, telXI coreX::URA3, Δnup133::kanMX4b | This work |
| YEF505 | MATα, ura3, leu2, ade2Δ, telXI coreX::URA3, SIR3-GFP::TRP1 | This work |
| YEF506 | MATα, ura3-52, trp1Δ63, ade2Δ, his3Δ200, SIR3-GFP::TRP1, Δhdf1::hphMX4 | This work |
| YEF508 | MATa, ura3-52, trp1Δ63, leu2Δ1, ade2Δ, SIR3-GFP::TRP1, Δnup84::kanMX4 | This work |
| YEF509 | MATa, ura3-52, trp1Δ63, leu2Δ1, ade2Δ, his3Δ200, SIR3GFP::TRP1, Δnup145C::kanMX4, telXI coreX::URA3 |
This work |
a
cs indicates cutting sites for the I-SceI endonuclease.
b
These strains are issued from matings with strains derived from BY4742 background (Brachmann et al., 1998); alleles for his3, ura3, leu2, lys2 are therefore not determined.