Table I. Effect of FA size on protein distribution.
| Protein | Shift | Antibody (provenance/company) |
|---|---|---|
| Stress fiber proteins | ||
| α-SMA | +++ | m (Skalli et al., 1986) |
| β-Cytoplasmic actin | + | m (clone 19B6; C. Chaponnier, University of Geneva, Geneva, Switzerland; unpublished data) |
| Total actin | ++ | m (clone Ac40; Sigma-Aldrich) |
| Caldesmon | − | rb (G. Pfitzer, University of Cologne, Cologne, Germany; Pfitzer et al., 2001) |
| Calponin | + | m (Sigma-Aldrich) |
| MLC | ++ | rb (Santa Cruz Biotechnologies, Inc.) |
| MLCK | + | m (clone K-36; Sigma-Aldrich) |
| NM-MHC (platelets) | ++ | rb (Benzonana et al., 1988) |
| SM-MHC (aorta) | − | rb (Benzonana et al., 1988) |
| SM22 | +++ | m (clone 1B8; S. Sartore, University of Padua, Padua, Italy) |
| Tropomyosin | + | rb (P. Gunning, Children's Hospital Westmead, Westmead, Australia; Percival et al., 2000) |
| Matrix adhesion proteins | ||
| β1 integrin | n.a. | rb (CHEMICON International, Inc.) |
| FAK | +++ | m (clone77; BD Biosciences) |
| Paxillin | − | m (clone177; BD Biosciences) |
| Phospho-FAK | n.a. | m (PY397; BD Biosciences) |
| Phospho-paxillin | n.a. | m (PY118; BD Biosciences) |
| Phospho-tyrosine | n.a. | m (clones PY69 and PY20; BD Biosciences) |
| Tensin | − | m (clone 5; BD Biosciences) |
| Vinculin | − | m (clone hVin-1; Sigma-Aldrich) |
| Others | ||
| GFP | n.a. | rb (Invitrogen) |
| Vimentin | − | m (clone V9; DakoCytomation) |
Summary of the antibodies used in this study to evaluate the redistribution of proteins from the cytoskeleton to the cytosolic fraction (Shift) after reducing adhesions from suFAs to classical FAs. MLCK, MLC kinase; m, mouse; n.a., not assessed; NM, nonmuscle; rb, rabbit; SM, smooth muscle.