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. 2006 Jan 16;172(2):295–307. doi: 10.1083/jcb.200507138

Table III. SNARE constructs.

Plasmid Chimera Vector Origin Marker Promoter
pJM88 His8-Sso1p pET24 ColE1 KAN T7
pJM90 Snc2p-His6 pET28a ColE1 KAN T7
BB442 GST-Sec9c pGEX-2T ColE1 AMP Ptac
pJM367 GST-Sso1p-ΔNRD pGEX-KG ColE1 AMP Ptac
pJM198 Sso1p (no tag) pRS316 CEN6 URA3 SSO1
pJM222 ΔNRD-Sso1p-HA pYX223 2μ HIS3 GAL1-10
pJM290 Sso1p-HA pYX223 2μ HIS3 GAL1-10
pJM291 Tandem t-SNARE without IHR pYX223 2μ HIS3 GAL1-10
pJM293 Tandem t-SNARE pYX223 2μ HIS3 GAL1-10
pJM311 Tandem t-SNARE–ΔNRD pYX223 2μ HIS3 GAL1-10
pJM334 Tandem t-SNARE pRS316 CEN6 URA3 SSO1
pJM414 Syn1ANRD-ΔNRD-Sso1p pYX223 2μ HIS3 GAL1-10
pJM427 Tandem t-SNARE pRS426 2μ URA3 SSO1
pJM429 [Q468R] Tandem t-SNARE–ΔNRD pYX223 2μ HIS3 GAL1-10