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. Author manuscript; available in PMC: 2007 Nov 6.
Published in final edited form as: Mol Genet Metab. 2007 Jul 17;92(1-2):137–144. doi: 10.1016/j.ymgme.2007.06.003

Fig 3. Responsiveness of IMFE1 cells to angiogenic growth factors and growth on Matrigel.

Fig 3

(A) Growth response curves of IMFE1 cells 72hs after stimulation with bFGF and VEGF. Results are expressed as a ratio of the absorbance of the stimulated wells to the absorbance of the non-stimulated wells. Data are presented as mean ± SE (n = 6). (*P<0.0003, #P<0.002, stimulated wells compared with non-stimulated wells, two-tailed Student’s t test) (B) eNOS expression analyzed by Western blot. IMFE1 cells were incubated with medium containing 0–100 ng/ml VEGF or bFGF for 48 hr. β-Actin was used as loading control. (C) Tube formation assay. IMFE1 cells grown on Matrigel extracellular matrix formed tube-like structures that were similar to those formed by primary HMVEC.