Table 1.

Conservation of stem cell aging in the systemic environment

	Rate of proliferation ESC/ASC (percentage of BrdU)	Call-fate identifier ESC (percentage of Oct4)/ ASC (percentage of Myf5)
10% young	59.5 ± 0.8, 59.3 ± 4.0	99.0 ± 0.1, 50.7 ± 9.5
10% old	32.7 ± 2.1, 27.3 ± 3.5	17.6 ± 3.2, 18.1 ± 5.9
5% young + 5% old	31.0 ± 2.6, 38.0 ± 2.0	20.6 ± 3.5, 17.1 ± 4.2

Quantified results from Figs 1, 2 are summarized and presented as mean percentages from experimental replicates ± SE. Rate of proliferation (BrdU) and cell-fate identifier (Oct4 or Myf5) are shown for both ESCs and ASCs cultured in heterochronic systemic conditions of 10% young (young), 10% old (old) or in 5%+ 5% mouse sera combination (young + old). Results for 5% young mouse sera are very similar to those for 10% young mouse sera and are shown in Fig. 1 (ASCs) and Supplementary Fig. S3 (hESCs).