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. 2007 Jan 1;176(1):11–17. doi: 10.1083/jcb.200605099

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Binding of JIP1 to Kinesin-1 is not sufficient for activation. (A) COS cell lysates expressing the indicated proteins were immunoblotted with antibodies to the Flag (top) or myc and HA tags (bottom). (B) The indicated lysates were immunoprecipitated (IP) with antibodies to the myc or Flag tags or no antibody as a control (−). Precipitates were immunoblotted with antibodies to the Flag tag (top) or myc and HA tags (bottom). (C) Prepolymerized taxol-stabilized MTs were added (+) or not added (−) to the indicated lysates with either ATP (T) or AMPPNP (N). After sedimentation, the MT pellets were immunoblotted with antibodies to the Flag (top) or myc and HA tags (bottom). Panels are from different parts of the same gel.