Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jan 1;176(1):11–17. doi: 10.1083/jcb.200605099

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — FEZ1 and JIP1 cooperate to activate Kinesin-1 in vitro. (A) COS cell lysates expressing the indicated proteins were immunoblotted with antibodies to the Flag tag (top) or to FEZ1 and the myc and HA tags (bottom). (B) The indicated lysates were mixed and immunoprecipitated (IP) with antibodies to KHC or the Flag tag or to no primary antibody as a control (−). Precipitates were immunoblotted with antibodies to the Flag tag (top), FEZ1 (middle), or myc and HA tags (bottom). (C) MT-binding assay as in Fig. 1 C. MT pellets were immunoblotted with antibodies to the Flag tag (top), FEZ1 (middle), or myc and HA tags (bottom). T, ATP; N, AMPPNP. (B and C) Panels are from different parts of the same gel. (D–F) Single-molecule motility assay. (D) Myc-KHC + 3xmCit-KLC lysates were mixed with lysates of mock-transfected cells (left) or cells expressing Flag-JIP1 and FEZ1-hsv (right). Representative motile events along Cy5-labeled MTs are shown in the kymographs (13 frames; 100-ms intervals). Bar, 1.0 μm. (E) Speed histogram. Myc-KHC + 3xmCit-KLC motors activated by JIP1 and FEZ1 move at a mean speed of 0.71 ± 0.31 μm/s (n = 47). Data are pooled from three separate experiments. (F) Single exponential decay fitting (red line) of the run length histogram shows that the same motors move processively for 0.46 ± 0.05 μm/run.