Skip to main content
. 2007 Jan 1;176(1):35–42. doi: 10.1083/jcb.200605135

Figure 1.

Figure 1.

Smurf1 is required for lamellipodia formation in carcinoma cells. (a) Cell extracts from MDAMB-231 cells transfected with control and Smurf1-directed siRNAs and shRNAs were immunoblotted for the indicated proteins. (b) Control or Smurf1 siRNA–silenced MDAMB-231 cells were stained for F-actin. (c) Control or Smurf1 shRNA–silenced MDAMB-231 cells were stained for F-actin. (d) In control and Smurf1-silenced MDAMB-231 cells, levels of total and active pull-downed, GTP-bound RhoA, as well as the total amount of Smurf1 and β-actin, were determined by immunoblotting. (e) Control and Smurf1-silenced MDAMB-231 cells were stained for F-actin and RhoA. Arrows point to RhoA staining in blebs. (f) Level of active Rho was evaluated in control and Smurf1-silenced cells by analyzing GFP fluorescence after addition of a RBD(rhotekin)-GFP probe that only binds active GTP-bound Rho, or addition of a control GFP probe. (g) Control and Smurf1-silenced MDAMB-231 cells were stained for ROCK1, p(S19)-, and p(T18/S19)-MLC2. Arrows point to examples of peripheral ROCK and phospho-MLC staining.