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. 2007 Jan 1;176(1):35–42. doi: 10.1083/jcb.200605135

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Smurf1 is required for tumor cell migration in 2D. (a) Random migration speed of control or Smurf1-silenced MDAMB-231 cells was averaged over a 6-h period of cell tracking by phase-contrast time-lapse microscopy. 10 μM Y-27632 or 0.5 μM tat-C3 was added to the medium 24 h before analysis. n = 3; mean ± the SD. At least 25 cells were analyzed in each experiment. P values of t test are indicated. (b) 48 h after transfection of p-EGFP-N1 alone or together with interfering Smurf1-C699A and Smurf1-ΔC2 mutants, EGF-expressing MDAMB-231 cells were tracked over a 6-h period by time-lapse microscopy for analysis of random migration. n = 2; mean ± the SD; at least 25 cells were analyzed in each experiment; P values of t test are indicated. (c) Alternatively, transfected cells were fixed and stained for F-actin and RhoA. Arrows indicate transfected cells.