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. 2006 Jan 30;172(3):347–362. doi: 10.1083/jcb.200507003

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — Agsic1Δ mutants arrest growth with abnormal nuclear distribution and spindle defects. (A) Agsic1Δ mutants (ASG65) were germinated in the presence of 50 μg/ml CloNAT to select for cells with the gene disrupted, were fixed and processed for antitubulin immunofluorescence, and compared with wild-type cells. The middle panels show the typical arrest phenotype of bipolar germlings, and the bottom panels show cells that arrest as a small mycelia and high nuclear density. Bars, 10 μm. (B) Higher magnification of nuclei showing multipolar spindle defects observed in Agsic1Δ cells. Nuclei are in blue, and microtubules are in green. Bar, 5 μm.