Table II.
A. gossypii strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| WT | leu2Δ thr4Δ | (Altmann-Johl and Philippsen,1996) |
| HHF1-GFP | pHHF1-GFP::GEN3 leu2Δ thr4Δ | (Alberti-Segui et al., 2001) |
| ASG38 |
SPC42-GFP::GEN3/SPC42 leu2Δ
thr4Δ |
This study |
| ASG43 | CLB1/2-13myc::GEN3 leu2Δ thr4Δ | This study |
| ASG46 | ade2::HHF1-GFP::ADE2 leu2Δ thr4Δ | This study |
| ASG50 |
ade2::HHF1-GFP::ADE2 leu2Δ
thr4Δ
SPC42-RFP::GEN3/SPC42 |
This study |
| ASG60 | CLN1/2-13myc::GEN3 leu2Δ thr4Δ | This study |
| ASG61 | SIC1-13myc::GEN3 leu2Δ thr4Δ | This study |
| ASG65 | sic1ΔNAT1 leu2Δ thr4Δ | This study |
| AKHAg19 | CLB1/2-NESa-13myc-GEN3 | This study |
| AKHAg22 | CLB1/2-NESi-13myc-GEN3 | This study |
| AKHAg26 | ade2::GFP-lacI-NLS::ADE2 | This study |
| AKHAg28 |
ade2::GFP-lacI-NLS::ADE2+placO-
CLB1/2-13myc |
This study |
| AKHAg29 | ade2::GFP-lacI-NLS::ADE2+placO-CLB1/2-13myc | This study |
All ASG and AKHAg strains are A. gossypii derived from the wild-type (ΔlΔt) strain. Individual nuclei are haploid, and most strains are homokaryotic (all nuclei have same genotype). In cases of heterokaryons (strains with mixed genotypes for a given locus), genotypes of the two different types of nuclei are written, separated by a plus sign.