Figure 5.
KIF14 is required for the localization of citron kinase but not other components of the central spindle or cleavage furrow. HeLa cells transfected with control or KIF14 siRNA duplexes for 48 h were fixed and stained with antibodies to the proteins (red) indicated in the figure. Actin was detected using 100 ng/ml rhodamine-phalloidin (red). DNA was stained with DAPI (blue). Bar, 10 μm. Arrows mark the positions of the cleavage furrow in control and KIF14-depleted cells stained for citron kinase.