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. 2006 Jan 30;172(3):383–393. doi: 10.1083/jcb.200507057

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Involvement of the IRE1–XBP1 pathway in the induction of Derlin-1 and -2 in response to ER stress. (A) IRE1α+/+, IRE1α−/−, XBP1+/+, and XBP1−/− MEFs were treated with 10 μg/ml tunicamycin (Tm) for the indicated periods. Total RNAs were isolated and analyzed by Northern blot hybridization using a DIG-labeled cDNA probe specific to mouse Derlin-2, Derlin-1, EDEM, BiP, or GAPDH. Closed and open arrowheads indicate the migration positions of 28S ribosomal RNA (4.7 kb) and 18S ribosomal RNA (1.9 kb), respectively. (B) 293T or HeLa cells were treated with 2 μg/ml tunicamycin (Tm) for the indicated periods. Total RNAs were analyzed as in A using a DIG-labeled cDNA probe specific to human Derlin-2, Derlin-3, EDEM, BiP, or GAPDH.