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. 2006 Jan 30;172(3):383–393. doi: 10.1083/jcb.200507057

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Effects of overexpression of Derlin-2 and -3 on degradation of NHK and NHK(QQQ). (A and B) HEK293 cells were mock-transfected or transfected with plasmid to express Derlin-2 or Derlin-3 tv2 tagged with the c-myc epitope at the respective NH₂ terminus together with plasmid to express NHK (A) or NHK(QQQ) (B). Transfected cells were pulse-chased and subjected to immunoprecipitation analysis using anti–α1-PI antibody as in Fig. 4 C. Migration positions of NHK, NHK(QQQ), Derlin-2, and Derlin-3 are indicated. The radioactivity of each NHK band was determined and expressed as relative to the summation of radioactivity of nine NHK bands obtained in each experiment. The relative radioactivity of each band was normalized with the value at chase period 0 h. The means from three independent experiments with SDs (error bars) are plotted against the chase period (bottom).