Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Feb 13;172(4):517–528. doi: 10.1083/jcb.200511071

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Immunofluorescence analysis of phenotypes induced by Dgrip75 depletion in S2 cells. (A) Localization of γ-TuSC components in mitotic cells. Control or Dgrip75-treated cells (Dgrip75_RNAi) were analyzed by staining with antibodies against γ-tubulin (a and c), Dgrip84 (e and g), and Dgrip91 (i and k). Merge (b, d, f, h, j, and l): blue, chromosomes; red, microtubules; green, γ-TuSC components. (B) Spindle pole focusing and localization of centrosomal markers. Dgrip75-depleted cells were stained with antibodies against Asp (a, green) or Cnn (b, green). Red, α-tubulin; blue, chromosomes. (C) Quantification of polar γ-tubulin by immunofluorescence. The maximal signal obtained after γ-tubulin staining was scored on each pole of bipolar spindles for control (closed bars) and Dgrip75-depleted cells (open bars). Correlations of 0.73 (n = 67; P95 = 0.62–0.84) and 0.72 (n = 69; P95 = 0.61–0.83), respectively, were observed between the maximal fluorescence measured at the two poles of control or treated cells. (D) Localization of γ-TuRC–specific components (Dgrip128, Dgrip163, and Dgp71WD) in mitotic cells. Blue, chromosomes; red, microtubules; green, γ-TuRC–specific components. Insets represent a hemispindle stained with antibodies against Dgrip128, Dgrip163, and Dgp71WD. For A and D, a quantitative view is shown in Table I. Bars, 5 μm.