Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Feb 13;172(4):529–540. doi: 10.1083/jcb.200507081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Bub3^+/−/Rae1^+/− MEFs have higher levels of SA molecular markers. (A) Western blot analysis of proteins in extracts from wild-type, Bub3^+/−, Rae1^+/−, and Bub3^+/−/Rae1^+/− MEFs at P3, P5, and P7. Blots were probed with antibodies against the indicated proteins. Note that the induction of senescence markers is delayed in Rae1^+/− MEFs; this delay was consistently seen in three independent Rae1^+/− MEF lines. (B) Western blots of protein extracts from P5 wild-type, Bub3^+/−/Rae1^+/−, and BubR1^H/H MEFs probed with the indicated antibodies. The results shown in A and B are representative for three independently generated MEF lines of each genotype. (C–H) Analysis of the fidelity of distinct DNA damage repair pathways by measuring cell survival and colony formation ability after exposing early passage MEFs to various kinds of DNA-damaging agents. (C–E) Survival curves of wild-type and Bub3^+/−/Rae1^+/− MEFs after DNA damage by γ-irradiation (C), UV type B (D), or paraquat (E). (F–H) Colony-forming assay of wild-type and Bub3^+/−/Rae1^+/− MEFs after DNA damage by γ-irradiation (F), UV type B (G), or paraquat (H). Error bars represent SD.