Table II.
Bub3/Rae1 double haploinsufficient MEFs develop more severe aneuploidy than BubR1 hypomorphic MEFs
| Mitotic MEF genotype (n) |
Mitotic figures inspected |
Percent aneuploid figures (SD) |
Karyotypes with indicated chromosome number
|
Percent mitotic figures with PMSCS (SD) |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 33 | 34 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 49 | 51 | 53 | ||||
| Wild type (3) | 150 | 9 (1) | 7 | 136 | 7 | <1 | ||||||||||||||
| Bub3+/− (3) | 150 | 19 (2) | 1 | 4 | 9 | 121 | 8 | 2 | 2 | 3 | 0 | |||||||||
| Rae1+/− (3) | 150 | 19 (2) | 5 | 2 | 6 | 121 | 9 | 1 | 2 | 4 | 0 | |||||||||
| Bub3+/−/Rae1+/− (3) | 150 | 41 (2) | 1 | 6 | 5 | 3 | 3 | 15 | 88 | 6 | 2 | 3 | 4 | 4 | 5 | 1 | 2 | 1 | 1 | 19 (3) |
| BubR1H/H (3) | 125 | 36 (6) | 5 | 13 | 80 | 20 | 6 | 1 | 15 (3) | |||||||||||
Empty spaces mean that there were no karyotypes with the indicated chromosome number. Karyotyping was performed at passage 5.