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. 2006 Feb 13;172(4):541–552. doi: 10.1083/jcb.200507101

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Recovery of an actin mutant unable to incorporate into filaments. Photobleaching experiment performed using cells transiently transfected with wild type GFP-actin (WT) or actin containing a point mutation at amino acid 62 (R62D). From left to right, the images represent prebleach, 1.5 s postbleach, 5 s postbleach, and 10 s postbleach. Dark colors represent lower concentrations of GFP, whereas bright colors represent higher concentrations, with white being the most concentrated. The bottom panel shows the FRAP recovery profiles of GFP-actin, GFP-actin + latrunculin, and the R62D actin mutant.