Table I.
FRAP recovery of fluorescent actins and fluorescent actin binding proteins
| Protein | Latrunculin | Percentage recovery | Standard deviation | P-value |
|---|---|---|---|---|
| Moesin-GFP | − | 91.3 | 4.7 | |
| + | 97.2 | 3.5 | ≤0.001 | |
| Anti-actin antibody | − | 78.5 | 4.8 | |
| + | 85.9 | 4.9 | ≤0.001 | |
| Anti–rabbit IgG | − | 94.2 | 1.8 | |
| + | 93.5 | 1.3 | NS | |
| GFP-actin (transient) | − | 84.7 | 4.5 | |
| + | 97.8 | 2.5 | ≤0.001 | |
| R62D actin–GFP | − | 99.3 | 1.4 | |
| GFP-actin (stable) | − | 84.0 | 9.4 | |
| FITC actin | − | 90.3 | 4.9 |
Percentage of recovery was measured at 11.5 s after photobleaching for each protein examined. In some instances, cells were preincubated for 60 min with latrunculin A. The P-value was obtained using a t test.