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. 2006 Feb 27;172(5):671–678. doi: 10.1083/jcb.200510061

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Expression of nonphosphorylatable cofilin S3A mutant promotes tight junction assembly in Par-3 KD MDCK cells. (A) MDCK cells were transfected with control, pSUPER–Par-3 (Par-3 KD), or pSUPER–Par-3 together with a construct expressing cofilin S3A (+cofilin S3A). Cells were subjected to calcium switch and stained for occludin at various times after calcium readdition. (B) Quantification of occludin cortical localization after calcium readdition. Asterisks denote a significant difference from Par-3 KD cells (P < 0.05) by t test. 6-h time point: control, n = 6; Par-3 KD, n = 7; cofilin S3A, n = 6. 7-h time point: control, n = 6; Par-3 KD, n = 7; cofilin S3A, n = 7. (C) Western blot analysis of total cell lysates from A. White lines indicate that intervening lanes have been spliced out. (D) Cofilin S3A expression partially rescues the development of transepithelial electrical resistance (TER) in Par-3 KD MDCK cells during calcium switch. MDCK cells were transfected with indicated constructs, and the kinetics of TER establishment was monitored for 13 h after the readdition of calcium. Each value is the mean of triplicate measurements (n = 3). Error bars represent SD.