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. 2006 Feb 27;172(5):671–678. doi: 10.1083/jcb.200510061

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Par-3 negatively regulates LIMK2 activity. (A) Par-3 inhibits LIMK2 kinase activity in vitro. HA-tagged LIMK2 or Par-3b was expressed in COS cells and immunoprecipitated. The immunocomplexes were subjected to in vitro kinase assays using GST-cofilin as the substrate or GST-Rac as a control. Coomassie brilliant blue (CBB) staining showed equal amounts of LIMK2 and GST-cofilin in the assays. (B) aPKC is not required for Par-3 to inhibit LIMK2 activity in vitro. HA-tagged Par-3c variant, which does not bind to aPKC, was used in the kinase assay together with Par-3b. (C) Ectopic Par-3 inhibits cofilin phosphorylation after LPA treatment. Control COS cells or cells expressing Par-3c were serum starved for 4 h and briefly treated with 5 μM LPA for indicated times. Total cell lysates were probed with specified antibodies. Numbers on the bottom of the blot indicate relative levels of phospho-cofilin normalized against the total cofilin level. Graph is representative of two independent experiments.