Figure 5.
Coiled-coil domains of Vta1 are required for function. (A) Schematic representation of Vta1 and mutant forms. Putative coiled-coil domains are indicated, as is the VSL region. (B) In vivo analysis of Vta1 coiled-coil mutants. BY4742 cells deleted for VTA1 were transformed with PrA-VTA1 chimeras as indicated, and MVB sorting was addressed by visualization of GFP-CPS.