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. 2006 Feb 27;172(5):771–782. doi: 10.1083/jcb.200512115

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Immunoblot analysis of zyxin-null fibroblasts. (A) Domains of zyxin include the α-actinin binding site, four ActA Ena/VASP binding repeats, two nuclear export signals (NES), and three LIM domains. Sites of epitopes for zyxin-specific antibodies 1D1 (zyxin aa 29–47) and B71 (zyxin aa 336–355) are identified by an asterisk. (B) Cell lysates of wild-type (+/+) and zyxin-null (−/−) fibroblasts were electrophoresed (10% SDS-PAGE) and either stained to confirm equal protein loading (Coomassie blue) or transferred to nitrocellulose for immunoblot analysis with anti-zyxin aa 29–47 (1D1) and anti-zyxin aa 336–355 (B71). (C–E) Immunoblot analysis of wild-type and zyxin−/− cells for expression of zyxin family members (C), zyxin-binding partners (D), and other focal adhesion proteins (E).