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. 2006 Feb 27;172(5):771–782. doi: 10.1083/jcb.200512115

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Copyright © 2006, The Rockefeller University Press

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Figure 10. — Jasplakinolide induces zyxin and VASP localization to actin filaments. Wild-type fibroblasts treated with jasplakinolide (JAS) or DMSO (control) were evaluated by indirect immunofluorescence microscopy. Zyxin localization in control fibroblast (A) and in jasplakinolide-treated fibroblast (B). (C) Phalloidin staining of F-actin in jasplakinolide-treated fibroblast. Jasplakinolide-treated fibroblast stained for caldesmon (D) and zyxin (E). (F) Caldesmon (pink) and zyxin (green) merge to white with colocalization. (G) Vinculin at focal adhesions in control fibroblasts colocalizes with zyxin (H) as seen in I (merge to white with colocalization). (J) Vinculin at focal adhesions in jasplakinolide-treated fibroblasts and zyxin (K) along actin filaments. (L) Merge (white) of vinculin (pink) and zyxin (green) signals shows the loss of colocalization upon jasplakinolide treatment. (M) Focal adhesion and ruffle localization of VASP in wild-type fibroblast. (N) VASP localizes along actin stress fibers in a jasplakinolide-treated wild-type fibroblast. (O) In a zyxin-null fibroblast, VASP fails to translocate to actin stress fibers in response to jasplakinolide.