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. 2006 Feb 27;172(5):771–782. doi: 10.1083/jcb.200512115

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Boyden chamber transwell assays demonstrate enhanced migration of zyxin-null fibroblasts. (A) Transwell migration (6 h) toward culture medium with 10% FBS on BSA or 20 μg/ml of collagen type I (Col I), collagen type IV (Col IV), fibronectin (Fib), laminin (Lam), superfibronectin (Sfib), or vitronectin (Vit). Data represent the mean ± SEM. n = 4. (B) Wild-type and zyxin-null cell migration with increasing fibronectin concentration (n = 4). (C) Transwell Matrigel invasion assays (24 h) of wild-type and zyxin-null fibroblasts, and zyxin-null cells expressing a GFP-zyxin transgene. (D) GFP-zyxin expressed in zyxin−/− fibroblasts localized properly to focal adhesions. (E) Immunoblot analysis of zyxin and GFP-zyxin. Immortalized cell lines were used for transwell migration assays. *, P < 0.05; **, P < 0.005.