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. 2006 Feb 27;172(5):771–782. doi: 10.1083/jcb.200512115

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Caldesmon protein in wild-type and zyxin-null fibroblasts. Fibroblasts were grown for 24 h in high (10% FBS) and low (0.5% FBS) serum, and then collected for immunoblot analysis. (A) Phospho-ser527/789-specific antibody detected caldesmon from both wild-type and zyxin-null cells, with a greater signal in high serum conditions (as expected for the phosphorylation-specific antibody). The caldesmon-specific immunoblot confirmed approximately equivalent levels of caldesmon in the lysates. (B) Incubation of fibroblast lysates with lambda-phosphatase followed by immunoblot analysis for caldesmon and paxillin. (C) His-tagged mouse l-caldesmon was expressed in wild-type and zyxin-null fibroblasts, and then evaluated by immunoblot analysis using his-specific and caldesmon-specific antibodies. (D) Immunoblot analysis of wild-type, zyxin-null, and zyxin-null cells expressing GFP-zyxin, with caldesmon- and zyxin-specific antibodies.