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. 2006 Mar 13;172(6):847–860. doi: 10.1083/jcb.200506119

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Generation of Separase and Securin floxed and Δ alleles. (A) Targeting strategy for Separase. Shown are the Separase genomic locus, the targeting vector, and the targeted allele. Exons are indicated as black boxes, the conserved peptidase domain starting at exon 18 (nucleotide 3980 of the mRNA) as red boxes, and the exon containing the conserved histidine and cysteine as a green box. For Separase the eight COOH-terminal exons were flanked by loxP sites (triangles). The selection cassette Neo-Tk is represented as red and green boxes and the DTA-cassette as a blue box. Cre-mediated recombination (dashed lines) was used to obtain Separase floxed or Δ alleles. (B) Southern blot to confirm germ-line transmission of Separase flox and Δ alleles with EcoRV-digested DNA and the internal probe c1. (C) Targeting strategy for generating Securin floxed and Δ alleles. (D) Southern blot to confirm germ-line transmission of Securin Δ alleles with BamHI-digested DNA using the internal probe c1.