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. 2006 Mar 13;172(6):847–860. doi: 10.1083/jcb.200506119

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Securin^Δ/ΔSeparase^Δ/+ embryos show developmental defects. (A) Securin ^Δ/+ Separase ^Δ/+ female mice were crossed with Securin ^Δ/+ Separase ^+/+ male mice. At 10.5 dpc, Securin ^Δ/Δ Separase ^Δ/+ embryos were obtained at the expected Mendelian ratio. However, Securin ^Δ/Δ Separase ^Δ/+ embryos were smaller and less developed than Securin ^Δ/+ Separase ^+/+ embryos. Bar, 1 mm. (B) Paraffin longitudinal sections of Securin ^Δ/+ Separase ^+/+ (a) and Securin ^Δ/Δ Separase ^Δ/+ (b) embryos 9.5 dpc were stained with Hoechst to visualize the nuclei. Squared regions in panels a and b are shown enlarged in panels c and d. Bars: (a and b) 100 μm; (c and d) 10 μm. Arrow points to a dead cell. (C) MEFs from Securin ^Δ/+ Separase ^+/+ and Securin ^Δ/Δ Separase ^Δ/+ embryos (10.5 dpc) were cultured and analyzed by immunofluorescence microscopy. Cells were stained with DAPI (a, d, g, and h) and α-tubulin (b and e). Bars, 10 μm. (D) Number of lobed nuclei in Securin ^Δ/+ Separase ^+/+ and Securin ^Δ/Δ Separase ^Δ/+ MEFs.

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