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. 2006 Mar 13;172(6):847–860. doi: 10.1083/jcb.200506119

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Hepatocytes after two thirds hepatectomy are highly polyploid. (A) Southern blot analysis of Separase^flox/flox and Separase^flox/floxMx-Cre livers before and 17 d after the two thirds hepatectomy. The Separase flox band is efficiently deleted in Separase^flox/floxMx-Cre livers. (B) Western blot from livers before, 48 h after, and 17 d after the two thirds hepatectomy. The Separase protein is detected in Separase^flox/flox hepatocytes, whereas in Separase^flox/floxMx-Cre hepatocytes the Separase protein is down-regulated. b.h., before the two thirds hepatectomy; a.h., after the two thirds hepatectomy. (C) Hematoxylin/ eosin staining of livers from Separase^flox/floxMx-Cre mice before and 17 d after the two thirds hepatectomy. Separase^flox/floxMx-Cre hepatocytes increase in ploidy and in cell size after the two thirds hepatectomy. Bar, 10 μm. (D) Single-cell DNA measurement of Feulgen-stained hepatocyte nuclei demonstrating the increase in DNA content in Separase^flox/floxMx-Cre hepatocytes at 17 d after the two thirds hepatectomy. (E) Hoechst staining of hepatocytes in culture. Compared with Separase ^flox/+ Mx-Cre hepatocytes, Separase^flox/floxMx-Cre hepatocytes demonstrate anaphases with sister chromatid missegregation, telophases with DNA bridges between daughter cells and multinucleated hepatocytes in interphase. Bar, 1 μm.

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