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. 2006 Mar 13;172(6):847–860. doi: 10.1083/jcb.200506119

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — iMEFs lacking Separase reveal higher ordered chromosomes. (A) Schematic overview of viral infection and harvesting procedure. (B) A portion of cells harvested for the analysis in C was methanol fixed, and their DNA content was analyzed by flow cytometry. Arrows indicate peaks showing <2C and >16C that were not present in the cells infected with AdGFP. (C) Chromosome spreads of cells harvested at the different time points. Before harvesting, cells were treated with nocodazole for 5 h to enrich mitotic cells and collected by mitotic shakeoff. (D) Higher magnification of the high-ordered chromosomes resulting from cells infected with AdCre and harvested at different time points. Number of chromatids is indicated. Control cells that underwent the same procedure except that they were not infected had a FACS profile very similar to that of the cells infected with AdGFP (not depicted). Bars, 10 μm.

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