Figure 6.

Cytokinesis occurs in some iMEFs lacking Separase. The infection and harvesting procedure was the same as in Fig. 5 A, except that a single time point 24 h after splitting was taken. (A) Immunostaining was performed using anti–Aurora B (red) and anti-MkLp1 (green) antibodies. DNA was stained with DAPI (blue). Prometaphase or metaphase (a), anaphase (b), and telophase (c) are shown. Arrows indicate unequal distribution of the DNA in cells infected with AdCre. (B) Different mitotic stages were scored in cells not infected with any virus (control) and cells infected with AdCre and -GFP, respectively. Mitotic stages were defined according to the Aurora B, MkLp1, and localization. n = 100 per cell type. (C) Immunostaining was performed using anti–cyclin B. DNA was stained with DAPI. (D) Cyclin B–positive and –negative metaphase plates were scored in cells infected with AdCre and -GFP. n = 100 per cell type. Bars, 10 μm.