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. 2006 Mar 27;172(7):1069–1079. doi: 10.1083/jcb.200508075

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Abilities of mutant β₂ chains to bind/phagocytose RBC and activate Rho. Cos-7 cells were cotransfected with wt α_M and mutant β₂ constructs as indicated, then challenged for 30 min with RBCs. The effect of expressing different β₂ constructs together with wt α_M on the attachment (A) and phagocytosis (B) of RBC was analyzed. Results are expressed relative to the values obtained for wt α_Mβ₂ (arbitrarily set to 100%). (C) After challenge with RBC for 20 min at 37°C, transfected Cos-7 cells were subjected to rhotekin pull-down assays. The fold-increase in Rho activity was calculated as for Fig. 1, with the ratio of GTP versus total Rho set to 1 for each β₂ construct in control conditions (i.e., in the absence of RBC). (A–C) Data shown are the mean ± SEM of at least three independent experiments.